The Coyote and Roadrunner zoom into view and begin to chase, freezing momentarily for the credits and Latin names to be shown: COYOTE: Famishus Vulgaris and ROAD RUNNER: Birdibus Zippibus. From here, Road speeds off, leaving the coyote to fall on the ground. Wiley recovers quickly, kicks up some dust, and begins to chase Road. However, Road, though, leaves so much dust in the road that his pursuer cannot see where he is going. Eventually, except for his ears, Wile E. is completely enclosed in the dust. Road pulls next to Wiley and beeps, alerting the coyote to his surroundings. Wile E.'s expression becomes foreboding, and the camera cuts out to show both apparently suspended in midair. As the dust clears, the coyote pokes his hands and feet through the bottom of the cloud, and then looks down to see nothing but air below him. The dust completely clears, showing that Road is perched on the edge of a cliff and the coyote on the wrong side of him. The poor coyote, once again, is subject to gravity. Determined not to let this happen again, Wiley climbs up a very high escarpment and surveys his surroundings for Road...who happens to have pulled up right behind him and now beeps such that the coyote falls back down. The camera zooms in on an obviously miffed Wiley as he falls to the ground again. Dusting himself off, the coyote gets up and walks out onto the road until Road beeps a second time and sends his rival directly into a low-slung rock plateau.
As he hasn't studied it enough, Wiley follows the instructions of The Art Of Roadrunner-Trapping: 1. Dig hole in road; 2. Camouflage hole; 3. Wait patiently; and 4. Eat Roadrunner. However, he never gets past the first step, as the jackhammer he uses vibrates enough to pull the coyote into the hole. When the power cord stretches enough to pull the plug out, the coyote climbs out and finds himself vibrating sporadically. To "punish" the book, Wile E. walks over to it and prepares to tear it in half, but one of the vibrations does the job for him.
Still trying to stop Road in his tracks, Wiley builds a brick wall on the mountain roads and waits. Soon, he hears Road braking in front of the wall. When his ears recover from the sonic assault and the dust cloud settles, he quizzically looks around the corner of the wall to see his own rear end. Wile E. makes random movements, which are imitated by his rear. After looking behind and ahead of him, Wile finally determines that this is a duplicate of himself and rolls dynamite under his "doppelganger"'s rear. The firework promptly explodes, and Wile laughs until he realizes it is his own tail on fire. He leaps directly into the air in pain and falls down to the ground.
Wile E., knowing about the "birds and the bees," leaves out bird seed for the Road Runner while he prepares to release a jar of bumblebees from a distance. But when Wile pulls the lid off, instead of attacking the munching Road next to them, the swarm flies 400 feet in the distance towards the coyote and repeatedly stings him.
Wile now prepares a second bird-seed trap, this time hoping to squash the flightless bird with an anvil. Of course, when Wile walks onto the board to drop the anvil, it breaks under his weight and falls towards Road, who simply steps to the side out of danger. The board halves fall on top of the resulting large hole to create a convenient bridge for Road back to his lunch.
Having had enough of the simple traps, Wile builds a long ramp and lights a bomb which should blow up Road when it gets to the bottom. But as soon as he lights the fuse, the bomb goes off.
Now, the coyote prepares a giant catapult in the road; however, the boulder is too heavy for the catapult to sling at the passing Road and flattens its owner.
Finally, Wile hopes to fire at Road with a harpoon. The rope, however, is around one of the coyote's feet, and drags the coyote's rear over a cactus and under several rocks, then directly into open space. Wile sees the pickle he is in and recovers in time to grab the very end of the rope. Unfortunately, the rope continues into a very thin pipe, then out onto the road and directly into a chicken race with a truck. Wile smashes directly through the truck, even, and then the spear finally impales a rock face, but this leads to the coyote being swung down into the path of a train and bumped all the way up to the edge of a precipice, ragged and exhausted while gasping for air and sobbing. Road pulls up directly behind him, but instead of beeping, which would possibly drive his opponent insane (and cause more gravity-induced humiliation), the flightless bird shows a soft side by holding up a sign saying: "I just don't have the heart." He dashes back the way he came, and the sign spins to "'Bye!"
The start of the cartoon (minus the title sequence and latin names) as well as the The Art of Roadrunner Trapping scene are used in The Bugs Bunny/Road Runner Movie.