The Looney Tunes Show has a variety of characters, classic and new, who appear or make cameos frequently in the show.
Bugs Bunny (voiced by Jeff Bergman) – Bugs Bunny lives a life of upper-middle-class suburban leisure, based on income from a popular Carrot Peeler that he invented. He lives in a well-appointed house, drives a compact car, and provides room and board for his friend, Daffy Duck. He spends his time watching sports on TV, hanging out with his friends and neighbors (mostly drawn from the classic Looney Tunes roster), and dating Lola Bunny (the latter under some protest). He generally plays the straight man to Daffy and Lola's various bouts of insanity, although he is not without his own quirks. He has exhibited somewhat compulsive/addictive tendencies, like having a high caffeine intake with coffee, becoming addicted to an energy drink that had dangerous chemicals in it, nearly leveled his own house in an increasingly deranged attempt to put up a shelf, and getting hooked on foods that contain butter. Despite being intelligent, he has shown some level of tomfoolery, such as when he found jail "a smart aleck's paradise" and mistook the Tasmanian Devil for a dog.
Daffy Duck (voiced by Jeff Bergman) – Daffy Duck is the roommate of Bugs Bunny. Unlike Bugs and their neighbors, Daffy has no way of earning money and relies on Bugs for food and shelter. He has tried on numerous occasions to get rich quick but ended up failing repeatedly. While Daffy's greed and jealousy of Bugs remains, it appears less antagonistic in the show. In the first episode, Bugs openly admits that Daffy is his best friend, despite his faults. Daffy has worked, and been fired from, numerous jobs due to negligence and/or incompetence. Despite this, however, Daffy has shown to be a very skilled hairdresser and successfully graduated beauty school. Daffy's three possessions that he is proud of are his (broken) blue recliner, his white collar which he always wears (revealed in some episodes to be a fine pearl choker), and his Papier-mâché parade float, constructed on top of a pickup truck, which is his main means of transport.
Porky Pig (voiced by Bob Bergen) – Porky Pig is one of Bugs Bunny and Daffy Duck's friends. Despite being bright and bookish, Porky has an innocent, naïve quality that Daffy frequently uses to his advantage, tricking Porky into parting with large sums of money or accompanying him in bizarre schemes. Porky originally worked a boring office job, but got fired following Bugs' example. He started his own catering company afterwards. In "Dear John", Porky was shown to have served on the city council. He starts a relationship with Petunia in the end of "Here Comes The Pig". In "Best Friends Redux", Daffy meets Porky's young self and ensures that he becomes good friends with Bugs & Rodney in their cabin, finally showing Porky an act of kindness.
Speedy Gonzales (voiced by Fred Armisen) – Speedy Gonzales is an extremely fast mouse who lives with Bugs and Daffy as their "mouse in the wall" and runs a pizza parlor called Pizzarriba. He is occasionally shown to act as Daffy's conscience. The episode "Sunday Night Slice" showed that Bugs bought his favorite restaurant Girardi's to prevent it from being closed and hired Speedy to help him. When Bugs decides he doesn't want to own a restaurant anymore, he hands ownership of it to Speedy who renames it "Pizzarriba".
Yosemite Sam (voiced by Maurice LaMarche) – Yosemite Sam is one of Bugs and Daffy's neighbors. He is a liar, a thief, and a cheat, amongst other things. Coming from a lower class background, he tends to steal Bugs' possessions, causing Bugs and Daffy to resent him.
Lola Bunny (voiced by Kristen Wiig) – Lola is Bugs' scatter-brained, bubbly, obsessive girlfriend, who has a habit of speaking rapidly, whether anyone else is listening or not. When they first meet, Bugs falls in love with her, but after learning how crazy and ditsy she is, he loses interest and often tries to escape her company. Lola develops a huge obsession with Bugs Bunny that he later finds creepy; Lola is never put off by Bugs' responses to behavior, that include taking photos of him in the shower, spying on him late at night, and often stalking him. Later in the series, however, Bugs eventually falls in love with Lola again which started when they went to Paris in the episode "Eligible Bachelors" and Bugs manages to stop her talking for a while. Her parents are members of a country club and her father likes Bugs so much that he considers him to be "The son he never had."
Tina Russo (voiced by Jennifer Esposito in season one, Annie Mumolo in season two) – A new character original to the show, it's Melissa Duck's rename, Tina Russo is a female duck who is Daffy's girlfriend. She works at a copy store called "Copy Place". Tina is another straight character of the show, with a no-nonsense personality. She first starts dating Daffy because "she likes a project"; she tolerates his selfish and arrogant behavior as she has a keen astuteness which allows her to read between the lines when they first meet; she works out that Daffy is actually insecure and jealous, and that his vain attitude is really a front. Daffy is amazed she works this out so quickly and later reveals Tina, through email, that he can't believe someone "so kind, beautiful, generous, and intelligent" would ever want to be with someone like him. After reading this, Tina is touched, and tells Daffy she loves him.
Tasmanian Devil (voiced by Jim Cummings) – In this show, the Tasmanian Devil is portrayed as walking on four legs like a real Tasmanian Devil and his eyes are bloodshot red (later turned yellow when Bugs uses a taming trick that Speedy Gonzales taught him). Initially, Bugs believed Taz to be a dog and kept him as a house pet much to Daffy's discomfort. Eventually, Bugs learned the truth and tried to return him to his home in Tasmania only to find out that Taz would rather live with him. When Taz is not causing trouble for Daffy, he has occasionally tried to eat Sylvester. In the episode "Ridiculous Journey," Taz spoke for the first time in the series and had bonded with Sylvester and Tweety while they evaded Blacque Jacques Shellacque.
Witch Lezah (voiced by Roz Ryan) – A witch who lives next door to Bugs Bunny and is often annoyed at the antics of Daffy Duck. Witch Lezah is also a hypnotherapist by trade. The character is very similarly based on Witch Hazel, with 'Lezah' being 'Hazel' spelled completely backwards.
Gossamer (voiced by Kwesi Boakye) – A large orange furry monster who is Witch Lezah's son. In stark contrast to previous characterizations, Gossamer is portrayed as timid and kind-hearted.
Emma Webster "Granny" (voiced by June Foray as an adult, Stephanie Courtney as young Granny) – An old lady who is one of Bugs Bunny's neighbors. Granny is revealed to have been a spy for the Allies in World War II. In "The Grand Old Duck of York," it is revealed that Granny teaches piano lessons.
Sylvester (voiced by Jeff Bergman) – Granny's Tuxedo cat who is always trying to devour Tweety, but always fails when Granny catches him and spanks him very hard to spit Tweety out.
Tweety (voiced by Jeff Bergman) – Granny's Yellow canary who is harassed by Sylvester. Tweety is revealed to have also been a spy for the Allies in World War II during Granny's youth.
Foghorn Leghorn (voiced by Jeff Bergman) – Foghorn Leghorn is a rich rooster who has worked under various jobs. He and Daffy get on very well, and are often involved in various schemes.
Pepé Le Pew (voiced by René Auberjonois in season one, Jeff Bergman in season two) – Pepé Le Pew is a skunk who's the local Casanova. In the episode "Members Only" he works as a wedding planner when he planned Bugs and Lola's wedding at the country club.
A direct-to-video film, Looney Tunes: Rabbits Run, was announced on April 30, 2015, with an accompanying trailer. It was released on August 4, 2015, though it was released earlier on July 7, 2015, through Walmart and Vudu.