An guffawing spider (voiced by Cy Kendall) spots his intended prey, a mute fly, on the ceiling, and indulges in various cat-and-mouse schemes to try to catch him for food, including painting a load of buckshot with "Kandy Kolor" and luring the fly to eat it and drawing him closer with a magnet, which only succeeds in attracting a set of metal cutlery which the spider has to dodge to save himself. Eventually, the spider catches his prey, and, when he is about to carve him up while singing the song "Would You Like to Take a Walk?", the fly points to a wall calendar giving the day as "Meatless Tuesday", a reference to food rationing during World War II. The frustrated spider runs to the United States Capitol and screams, "You can't do this to me! You can't, you can't!"
The title is a pun on Meatless Friday, a reference to the traditional Roman Catholic practice of abstinence from meat on Fridays.
Cy Kendall, who voices the Spider, based his performance on Tex Avery's voice. It was so good that, years later, Avery became convinced that he had done the voiceover.
This is the first cartoon to use the finalized MERRIE MELODIES logo on the closing rings, which would be used until the end of 1955. This is also the first cartoon to use the 1944 rings evident from blue rings and red background.
Bugs Bunny is mentioned once by the spider when the fly does air acrobatics to heckle him as "a poor man's Bugs Bunny".
Some prints of the USA dubbed version, for reasons unknown, have a subtle edit to remove a black screen set in-between the part where the Spider laughs as he says "Well that's one on me. Caught me off my guard" after the fly releases the spider from the sugar cube trap and the part where the fly does acrobatics in the air to heckle the spider. The EU 1995 Turner dubbed version prints however, does not have this edit. However, some USA dubbed prints of this cartoon also exist uncut.